Thursday, June 22, 2006

Transformation for Expression

Wednesday afternoon I transformed approx. 1 ng of a plasmid miniprep from colony culture #31 into Novagen's Tuner(DE3)pLacI. After a 60 minute outgrowth step (37 degrees with axial shaking - I don't have access to an orbital shaker presently), cells were spread on LB plates containing 1% glucose, chloramphenicol and carbenicillin and grown overnight.


Post a Comment

<< Home