Wednesday, April 19, 2006

Preparation of Yeast Genomic DNA

Epicentre MasterPure Yeast DNA Purification Kit.

Procedure overview:
1. Lyse yeast cells in the Yeast Cell Lysis Solution (non-enzymatic).
2. Precipitate and remove proteins using MPC Protein Precipitation Reagent.
3. Precipitate, wash, and resuspend the nucleic acids.

Used 1.3 ml A600 = 12/ml. Yield was 816 ng/ul.


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